Journal: International Wound Journal
Article Title: Characterisation of dehydrated amnion chorion membranes and evaluation of fibroblast and keratinocyte responses in vitro
doi: 10.1111/iwj.13103
Figure Lengend Snippet: Multiplex enzyme‐linked immunosorbent proteomic microarray evaluation of dACM grafts and evaluation of dACM protease inhibition of MMP‐2 and MMP‐9 in vitro. dACM grafts from 15 human donors were assessed for 25 targets relevant to native wound healing. Results shown here are categorised into groups (A) angiogenic growth factors, (B) regenerative growth factors, (C) immune‐modulating factors, and (D) matrix proteins. Reduction of MMP‐2 activity (E), and reduction of MMP‐9 activity (F) with the addition of dACM. Average ± SD, ◊ denotes P < 0.001. Abbreviations: dACM, dehydrated amnion/chorion membranes; MMP, matrix metallopeptidase; NNGH, N‐Isobutyl‐N‐(4‐methoxyphenylsulfonyl)glycyl hydroxamic acid
Article Snippet: Briefly, a custom quantitative multiplex enzyme‐linked immunosorbent assay (ELISA) proteomics microarray (Raybiotech Inc, Norcross, Georgia) was used to quantify growth factors and cytokines within dACM.
Techniques: Multiplex Assay, Microarray, Inhibition, In Vitro, Activity Assay